Protein Analysis
Division 1.5
Proteins are the most important and diverse class of substances in medicine, bioengineering and food analysis, constituting one of the greatest challenges in analytical chemistry. In the pharmaceutical industry, most new developments are based on proteins that generate revenues in the billions and enable treatments of formerly incurable diseases. In foods, the protein content is one of the most important nutrient components.
The Protein Analysis division concerns itself with the development and establishment of precise and reliable methods to detect protein content as a sum parameter for industrial and research applications. We also work on methods for the highly selective analysis of individual or multiple parameters in extremely complex mixtures, for instance biomarkers in blood plasma to enable safe, cost-efficient diagnostics, e.g. for SARS-CoV-2.
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Fields of expertise
- Immunoassays and antibody development
- Bioconjugation and protein immobilisation
- Quantitative protein analysis
- Affinity chromatography
- Monolithic columns
- Plant viruses as nanoplatforms
- Synthetic peptide libraries and screening methods
- Biosensors
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Main activities
- Development of novel affinity methods for the extraction and concentration of proteins from extremely complex samples (e.g. blood serum, cell culture supernantants, homogenates)
Design of new affinity binders - Chromatographic and mass-spectrometric analysis of amino acids, peptides and proteins
- Quantification of proteins
- Development and application of new monolithic affinity materials
- Development of reference materials/methods in relation to peptides/proteins
- Optimisation of affinity sensors
- Screening of biomarkers
- Plant viruses as nanomaterials
- Development of novel affinity methods for the extraction and concentration of proteins from extremely complex samples (e.g. blood serum, cell culture supernantants, homogenates)
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Range of services/technical equipment
- HPLC, Nano-LC, LC-LC hyphenation
- ESI-HRMS (Orbitrap)
- MALDI-MS/MS
- Affinity enrichment, affinity extraction
- Antibody design, immunoassay development
- Surface acoustic wave sensor (SAW)
- Low-density microarrays
- High-throughput screening
- Synthesis of new haptens
- Synthesis of innovative carriers
- Glass-based monolithic columns
- Derivatization of proteins / peptides / amino acids
- Peptide synthesis and OBOC peptide libraries
- Protein hydrolysis
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Publications of the division
In the database PUBLICA you will find publications by BAM employees.
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Dr. rer. nat. Michael G. Weller
